The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog OPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog OPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog OPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog OPG in the samples is then determined by comparing the OD of the samples to the standard curve.
*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
At a glance
- Reactivity: Dog
- Sample types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Source: manufacturer product information, Assay Genie.
