There are two kinds of antioxidant system, one is enzyme antioxidant system, including superoxide dismutase (SOD),) catalase (CAT), glutathione peroxidase (GSH-Px). The other is non-enzymatic antioxidant systems, including uric acid, vitamin C, vitamin E, glutathione, bilirubin, α-lipoic acid, carotenoid. Antioxidant capacity is thought to be the cumulative effect of all antioxidants in blood and body fluids.
The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.
At a glance
- Sample types: serum, plasma, urine, saliva, tissue, cells
- Storage: -20°C
Source: manufacturer product information, Assay Genie.
